The Department of Communicable Disease Control of the Ministry of Health of Lao People's Democratic Republic (Lao PDR) organized a Regional Workshop on Implementing the Asia Pacific Strategy for Emerging Diseases and Public Health Emergencies (APSED III) in the Greater Mekong Subregion (GMS) on 19 to 20 November 2018 in Pakse, Champasack Province, Lao PDR (under L3465/G0516-Lao: GMS Health Security Project). The objective of the workshop was to assess the implementation aspects of APSED III for GMS countries to enable improved national and regional planning and knowledge for emerging infectious diseases and implementation strategies for enhanced health security. Senior personnel and specialists from Ministries of Health of Cambodia, Lao PDR, Thailand, and Vietnam, including representatives from various APSED/International Health Regulations related sectors (i.e., laboratory, surveillance and response, zoonoses, policy, and risk communication) participated in the workshop.